Wipe the stoppers with alcohol
Use 70% isopropyl alcohol and let dry for 10–15 seconds.
Technical guide
Reconstitution of lyophilized peptides
Reference tool and handling procedure for RUO scientific research.
Interactive calculator
- Concentration
- 5000 mcg/ml
- Volume
- 0.050 ml
- U-100 syringe
- 5.0 u
Mathematical reference tool for RUO use. Peptide Life does not recommend dosing or human use; consult scientific literature and approved protocols.
Step-by-step procedure
Draw BAC water
Draw the calculated volume of bacteriostatic water using sterile material.
Inject onto the wall
Slowly inject the solvent onto the inner wall — do not aim directly at the lyophilized cake.
Wait and swirl gently
Wait 30–60 seconds and swirl the vial. Do not shake vigorously.
Verify dissolution
The solution must appear clear and free of visible particles.
Label and refrigerate
Record date and concentration; store at 2–8°C per protocol.
Frequently asked questions about reconstitution
+What type of water is used for reconstitution?
Sterile bacteriostatic water with 0.9% benzyl alcohol. For short-term analysis, sterile water may be used under the validated protocol.
+How is a reconstituted solution stored?
Label with date and concentration and refrigerate at 2–8°C, avoiding vigorous agitation and repeated freeze-thaw cycles.
+Does the calculator recommend dosing?
No. It only converts units for RUO technical work; it does not provide therapeutic instructions or human-use recommendations.